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Calpain 1 is a cysteine-protease existing as a heterodimer made up of a large and small subunit. The large subunit is 714 residues long (mass 81.9 kDa) with a pI of 5.49. The small subunit is 268 residues long (mass 28.3 kDa) with a pI of 5.05. Calpain 1 is inhibited by calpastatin, an intrinsically disordered protein of 708 residues (mass 76.5 kDa) with a pI of 4.97. Suggest a purification method that would allow you to separate calpain 1 from calpastatin. Describe conditions and explain why the chosen method would work and give reasons why it wouldn’t work.


Explain how the nucleus, mitichondria, ribosome, endoplasmic reticulum, and Golgi body work to produce lysosomes within a macrophage to enable it to fight infection


1. What is the difference between integumentary system and exoskeleton?

Hair, feather, scales, nails and horns are examples of exoskeleton or integumentary (because some website consider them as a part of integumentary system while some consider them as exoskeletal features

https://medical-dictionary.thefreedictionary.com/exoskeleton

https://byjus.com/biology/difference-between-endoskeleton-and-exoskeleton/

).

2. Human nails are exoskeletal features or not?

3. Human skeletal system is entirely an endoskeleton or italso has some exoskeletal features?


My first nucleotide is Guanine.how to label all the components with proper atoms and numbering system.


Thanks in advance


Name the meristem part of a plant. 


In media preparation, the culture media needs to be adjusted to ± pH 5.8. Give reasons why it is so and what would happen if the pH is lower than 5 or more than 6.5? 


Generally, a plant tissue culture laboratory consists of 8 sections. Based on the knowledge that you have learned in each section, propose a drawing plan on how are you going to compartmentalize them, and state your reasons. 


In plant media culture, list the function(s) for each element present in “minor inorganic nutrients” and iron. 


REPLICATION FORK.

Question 1 . If the gene for primase is mutated, what part of the replication will be affected?


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