Question

In media preparation, the culture media needs to be adjusted to ± pH 5.8. Give reasons why it is so and what would happen if the pH is lower than 5 or more than 6.5? 

Answer 1

Apart from complete nutritional composition, right and stable pH is another important requirement for optimum microbial growth in culture media. The pH of a culture medium should be suitable to the microorganisms that will be grown. If pH will exceed the allowable limits, the microbial growth can be impared.