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Demonstrate how you could isolate and culture Pseudomonas putida from soil sample contaminated with hydrocarbons
1. The application of organic matter to the soil improves soil quality. Discuss

2. i. Describe the structure of any two tectosilicates ,minerals found in soils.

ii. State the impact of these minerals on soils.

3. Isomorphous substitution is mechanism for the creation of charges on soil minerals. Explain
Actinomycetes are usually classed within the same taxonomic family as bacteria but in many aspects of their morphology and growth patterns resemble fungi. Explain.
Horizontal gene transfer is made possible by the existence of mobile genetic elements, such as plasmids (extrachromosomal genetic material), transposons (“jumping genes”) and bacteria-infecting viruses (bacteriophages). These elements are transferred between organisms through different mechanisms, which in prokaryotes include transformation, conjugation, and transduction.
Finally, discuss the danger that antibiotic resistance poses in today’s society, consider how man has contributed to this and suggest any strategies that you think may be able to halt it or prevent its expansion
As with all proteins, beta-lactamase is encoded by a section of DNA – but how does that DNA and the ability to produce a new protein transfer from one population of bacteria that have resistance to another population that don’t?

Other than direct transfer from parent to daughter cell, horizontal transmission of DNA between different genomes also occurs. Horizontal gene transfer is made possible by the existence of mobile genetic elements, such as plasmids (extrachromosomal genetic material), transposons (“jumping genes”) and bacteria-infecting viruses (bacteriophages). These elements are transferred between organisms through different mechanisms, which in prokaryotes include transformation, conjugation, and transduction.



Write a sentence for each of these mechanisms describing the manner in which the DNA can be transferred from one cell to another.
What are some things that can damage a farmer’s crops?





What can farmers do to protect their crops?
Antibiotics are used to treat a wide variety of bacterial infections and have saved millions of lives since they were first introduced in the 1940s and 1950s. However, due to both overuse and misuse, many are no longer effective and The World Health Organisation (WHO) considers the emergence of new antibiotic-resistant bacteria to be a serious threat to global public health.

According to their mechanism of action, antibiotics can be divided into three main groups, those inhibiting cell wall synthesis, those inhibiting protein synthesis and those that inhibit nucleic acid synthesis.

Write a sentence for each of these mechanisms describing the manner in which the DNA can be transferred from one cell to another.
A useful devise in predicting possible offspring
Without the vital process of the biological activity of soil biota, the soil would become a respiratory of dead plant remains. True/False. Discuss your answer.
Discuss the role of the soil flora and fauna in relation to soil quality and productivity.
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