Biology Answers

A 17 year old female has 4 days history of fever. Dengue NS1 Ag was requested and revealed:

Dengue NS1 Ag = positive

Questions:

a. Can you conclude from the result that the patient has active dengue infection? Can you conclude from the result that the patient does not have dengue at the moment? Explain your answer.

b. What will be your advise to the patient?

A 5 year old boy has been having fever for 2 days. Dengue IgM and IgG were requested and revealed: IgM = negative

IgG = positive

Question: What will be your advice to the parents?

A 5 year old boy has been having fever for 2 days. Dengue IgM and IgG were requested and revealed: IgM = negative

IgG = positive

Question: Can you conclude from the result that the patient has dengue? Can you conclude from the result that the patient does not have dengue at the moment? Explain your answer.

The DNA sequence for Species A is mutated to: GAC-TGA-CTC-CAC-TGG. What is the effect, if any, on the protein produced?

Evaluate the clonal selection hypothesis in terms of autoimmunity and

immunological memory.

in agarose gel electrophoresis, low signal intensity was observed in the mixture of different molecular weight molecules. why is this so

Study the floral dissection of clivia miniata,record the floral features and compile a floral formula.

pattern and profile used in the sequence analysis for functional assignment

Here are two motifs. Which of the following matches them?

[QAPRG]-X-D-Q-X-{RT}-X-S

A. QQQQQQQS

B. PPDQDDDS

C. LLDQQLLS

D. QQDQQTQS

E. GGDQGGGS

G-[AWEE]-X- X-{EGR}-X-{WE}

A. GGEPRGP

B. GAAAAGG

C. GWEPAGG

D. GWEPAGP

E. GREETLP

Part B. PDB Files. 5 Marks

1. Go to the PDB home page,

http://www.rcsb.org/pdb/ . Retrieve the 3NSJ file. What is the

protein that is the subject of this file, and from what species?

2. Why do you think the authors wanted to determine the structure of this protein?

3. Describe some of the insights into the function of the protein the structure gave.

4. What experimental technique was used to collect the data? What is the resolution of the

derived structure (in angstroms)

5. Approximately how many alpha helices and beta sheets does the protein contain?

1. For the sequence below, answer the following questions.

a) Identify the protein and species

b) At approximately what amino acid number is the most hydrophilic region

of the protein?

Use the default window size for the tool you use.

You

must state which tool/program you have chosen to use.

c) Do you expect it to be secreted? If so, by what mechanism?

d) List the motifs that are identified. Use the standard parameters for the tool

you choose to use.

Seq 4

MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPTRKERFTVLISPHVN

KDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISLG

a)

b)

c)

d)