1. For the sequence below, answer the following questions.
a) Identify the protein and species
b) At approximately what amino acid number is the most hydrophilic region
of the protein?
Use the default window size for the tool you use.
You
must state which tool/program you have chosen to use.
c) Do you expect it to be secreted? If so, by what mechanism?
d) List the motifs that are identified. Use the standard parameters for the tool
you choose to use.
Seq 4
MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPTRKERFTVLISPHVN
KDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISLG
a)
b)
c)
d)
a) 30S ribosomal protein S10; Escherichia coli
b) Approximately 60 aa (Expasy ProtScale)
c) No, it does not contain a signal sequence. On the other hand, ribosomal proteins are cytoplasmic
d) 30S ribosomal protein S10p/S20e
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