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Why IPTG is used for expression studies than lactose/ allolactose in E. coli?a. Concentration remains constant


The high copy number plasmid is isolated from 2 mL of E. coli cells containing 4 × 108 CFU/mL and it is quantified as 120 ng/µL using nanospectrophotometer. Size of the plasmid is 5400 kb. Convert the concentration of plasmid from ng to pM concentration.

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15. A researcher is working with haemoglobin that contains subunits of differing molecular weights. If the researcher performs SDS-PAGE, how many distinct bands should he see on the gel?


A researcher isolates DNA using TBE buffer and use it for cloning experiment. He fails to get the recombinant plasmid, why?


The receptor for aldosterone appears to have evolved much earlier than aldosterone itself. Propose a hypothesis to explain how this might have occurred.
Explain how glucocorticoid secretion varies during a 24-hour period.
Describe the actions and regulation of the adrenal hormones, including their role in helping the body respond to stress
How have the roles of cortisol and corticosterone changed among the various major groups of vertebrates? What features are more or less common to all?

Which portion of the host cell makes it susceptible to a viral attack?



An average egg production of a Flock is 200 eggs per year, Sire birds with average breeding value of 300 eggs were Selected. (Assume as if Heritability of egg production and generation interval is 0.3 and eight months respectively. For a given Flock of Birds determine : A) Genetic gain per generation.

B) Genetic Gain per year.

C) Average egg Production in next Generation.


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