Answer to Question #214705 in Molecular Biology for Pinky

Question

The high copy number plasmid is isolated from 2 mL of E. coli cells containing 4 × 108 CFU/mL and it is quantified as 120 ng/µL using nanospectrophotometer. Size of the plasmid is 5400 kb. Convert the concentration of plasmid from ng to pM concentration.

Your answer

Answer 1

A high-copy plasmid should yield between 3-5 ug DNA per 1 ml LB culture, while a low-copy plasmid will yield between 0.2-1 ug DNA per ml of LB culture.