Biology Answers

CS444: BIOINFORMATICS (Assignment 1 - Lab)

(To be made handwritten)

The following transcript was found to be abundant in a human patient’s blood sample.

>Example

ACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC

AAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC

TGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGG

CCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTG

TCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT

GCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAA

GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTGGCCATGCTT

CTTGCCCCTTGGGCCTCCCCCCAGCCCCTCCTCCCCTTCCTGCACCCGTACCCCCGTGGTCTTTGAATAA

AGTCTGAGTGGGCGGCA

Q1:

Which BLAST program should we use in this case?

Sol:

Q2:

What are the names and accession numbers of the top ten hits from your BLAST search?

Sol:

Q3:

What are the percent identities for the top five hits?

Sol:

Q4:

How many identical and non identical nucleotides are there in your top hit compared to your last reported hit?

Sol:

Q5:

What is the “Official Symbol” and “Official Full Name” for this gene?

Sol:

Q6:

What is the “Lineage” for this gene?

Sol:

Q7:

What chromosome is this gene located on?

Sol:

Q8:

How many exons are annotated for this gene?

Sol:

Q9:

What is the function of the encoded protein?

Sol:

Q10:

Does the protein have a role in human disease(s)? If so, what diseases?

Sol:

CS444: BIOINFORMATICS (Assignment 1)

Q1: What is the complement to the DNA sequence given below?

5’-ACCAAACAAAGTTGGGTAAGGATAGATCAATCAATGATCATATTCTAGTACACTTAGGATTCAAGATCCT

ATTATCAGGGACAAGAGCAGGATTAGGGATATCCGAGATGGCCACACTTTTGAGGAGCTTAGCATTGTTC

AAAAGAAACAAGGACAAACCACCCATTACATCAGGATCCGGTGGAGCCATCAGAGGAATCAAACACATTA

TTATAGTACCAATTCCTGGAGATTCCTCAATTACCACTCGATCCAGACTACTGGACCGGTTGGTCAGGTT

AATTGGAAACCCGGATGTGAGCGGGCCCAAACTAACAGGGGCACTAATAGGTATATTATCCTTATTTGTG

GAGTCTCCAGGTCAATTGATTCAGAGGATCACCGATGACCCTGACGTTAGCATCAGGCTGTTAGAGGTTG

TTCAGAGTGACCAGTCACAATCTGGCCTTACCTTCGCATCAAGAGGTACCAACATGGAGGATGAGGCGGA

CCAATACTTTTCACATGATGATCCAAGCAGTAGTGATCAATCCAGGTCCGGATGGTTCGAGAACAAGGAA

ATCTCAGATATTGAAGTGCAAGACCCTGAGGGATTCAACATGATTCTGGGTACCATTCTAGCCCAGATCT

GGGTCTTGCTCGCAAAGGCGGTTACGGCCCCAGACACGGCAGCTGATTCGGAGCTAAGAAGGTGGATAAA

GTACACCCAACAAAGAAGGGTAGTTGGTGAATTTAGATTGGAGAGAAAATGGTTGGATGTGGTGAGGAAC

AGGATTGCCGAGGACCTCTCTTTACGCCGATTCATGGTGGCTCTAATCCTGGATATCAAGAGGACACCCG

GGAACAAACCTAGGATTGCTGAAATGATATGTGACATTGATACATATATCGTAGAGGCAGGATTAGCCAG

TTTTATCCTGACTATTAAGTTTGGGATAGAAACTATGTATCCTGCTCTTGGACTGCATGAATTTGCTGGT

GAGTTATCCACACTTGAGTCCTTGATGAATCTTTACCAGCAAATGGGAGAAACTGCACCCTACATGGTAA-3’

Q2: What is the mRNA sequence of the given DNA sequence in Q1?

Q3: What is the protein sequence formed from the mRNA sequence of Q2?

Q4: What will be the mRNA encoded sequence if all the “AT”s are mutated into “TA”s in Q1 DNA sequence?

Q5: What will be the protein sequence of the new mRNA sequence formed after Q4?

Jessica was in the hospital for an extended period with a severe respiratory infection. During that period she received multiple IM injections and was discharged with orders to continue the injections daily until the doctor advised her to discontinue them. The nursing staff had been injecting the medication into the gluteal area, but this area was becoming sore from all of the prior injections. What options might she use for the future to avoid pain in the area of the injections?

Student 

Identify and explain the joints and muscles (lower body)and movements involved in cycling. Focus on the power phase and the recovery phase.

identify and explain the muscles and joints (upper body) and movements involved in swimming. Focus on the power phase and the recovery phase.

Explain why the potato in 0.0mol/dm^3 sugar solution increases in mass. (question about the effect of different concentrations of sugar solution on pieces of potato).

compare and contrast the environment of the chicken and the environment of the t rex

List four vitamins and four minerals with their i) major functions, ii) sources and, iii) signs of deficiency.

1. Create a “formula” for the process you used above, such that you’d be able to determine the field of view for a lens of any magnification if you knew the field of view of another lens magnification on the same microscope.
2. Using your answers from the previous two questions, estimate the size -- in microns -- of an organism if it covered one-third of the field of view at 100X total magnification. Show your work/process.

Which of the following includes all the others

a. Phylum

b. order

c. Family

d. Genus

e. Species

What are the muscle movements involved in cycling and explain the joints only the lower body?