Answer to Question #158279 in Bioinformatics for M Umair Naseer

Question

CS444: BIOINFORMATICS (Assignment 1)

 

 

Q1: What is the complement to the DNA sequence given below?

 

5’-ACCAAACAAAGTTGGGTAAGGATAGATCAATCAATGATCATATTCTAGTACACTTAGGATTCAAGATCCT

ATTATCAGGGACAAGAGCAGGATTAGGGATATCCGAGATGGCCACACTTTTGAGGAGCTTAGCATTGTTC

AAAAGAAACAAGGACAAACCACCCATTACATCAGGATCCGGTGGAGCCATCAGAGGAATCAAACACATTA

TTATAGTACCAATTCCTGGAGATTCCTCAATTACCACTCGATCCAGACTACTGGACCGGTTGGTCAGGTT

AATTGGAAACCCGGATGTGAGCGGGCCCAAACTAACAGGGGCACTAATAGGTATATTATCCTTATTTGTG

GAGTCTCCAGGTCAATTGATTCAGAGGATCACCGATGACCCTGACGTTAGCATCAGGCTGTTAGAGGTTG

TTCAGAGTGACCAGTCACAATCTGGCCTTACCTTCGCATCAAGAGGTACCAACATGGAGGATGAGGCGGA

CCAATACTTTTCACATGATGATCCAAGCAGTAGTGATCAATCCAGGTCCGGATGGTTCGAGAACAAGGAA

ATCTCAGATATTGAAGTGCAAGACCCTGAGGGATTCAACATGATTCTGGGTACCATTCTAGCCCAGATCT

GGGTCTTGCTCGCAAAGGCGGTTACGGCCCCAGACACGGCAGCTGATTCGGAGCTAAGAAGGTGGATAAA

GTACACCCAACAAAGAAGGGTAGTTGGTGAATTTAGATTGGAGAGAAAATGGTTGGATGTGGTGAGGAAC

AGGATTGCCGAGGACCTCTCTTTACGCCGATTCATGGTGGCTCTAATCCTGGATATCAAGAGGACACCCG

GGAACAAACCTAGGATTGCTGAAATGATATGTGACATTGATACATATATCGTAGAGGCAGGATTAGCCAG

TTTTATCCTGACTATTAAGTTTGGGATAGAAACTATGTATCCTGCTCTTGGACTGCATGAATTTGCTGGT

GAGTTATCCACACTTGAGTCCTTGATGAATCTTTACCAGCAAATGGGAGAAACTGCACCCTACATGGTAA-3’

 

Q2: What is the mRNA sequence of the given DNA sequence in Q1?

 

Q3: What is the protein sequence formed from the mRNA sequence of Q2?

 

Q4: What will be the mRNA encoded sequence if all the “AT”s are mutated into “TA”s in Q1 DNA sequence?

 

Q5: What will be the protein sequence of the new mRNA sequence formed after Q4?

 

Answer 1

Q1: Complement sequence:

3'-TGGTTTGTTTCAACCCATTCCTATCTAGTTAGTTACTAGTATAAGATCATGTGAATCCTAAGTTCTAGGATAATAGTCCCTGTTCTCGTCCTAATCCCTATAGGCTCTACCGGTGTGAAAACTCCTCGAATCGTAACAAGTTTTCTTTGTTCCTGTTTGGTGGGTAATGTAGTCCTAGGCCACCTCGGTAGTCTCCTTAGTTTGTGTAATAATATCATGGTTAAGGACCTCTAAGGAGTTAATGGTGAGCTAGGTCTGATGACCTGGCCAACCAGTCCAATTAACCTTTGGGCCTACACTCGCCCGGGTTTGATTGTCCCCGTGATTATCCATATAATAGGAATAAACACCTCAGAGGTCCAGTTAACTAAGTCTCCTAGTGGCTACTGGGACTGCAATCGTAGTCCGACAATCTCCAACAAGTCTCACTGGTCAGTGTTAGACCGGAATGGAAGCGTAGTTCTCCATGGTTGTACCTCCTACTCCGCCTGGTTATGAAAAGTGTACTACTAGGTTCGTCATCACTAGTTAGGTCCAGGCCTACCAAGCTCTTGTTCCTTTAGAGTCTATAACTTCACGTTCTGGGACTCCCTAAGTTGTACTAAGACCCATGGTAAGATCGGGTCTAGACCCAGAACGAGCGTTTCCGCCAATGCCGGGGTCTGTGCCGTCGACTAAGCCTCGATTCTTCCACCTATTTCATGTGGGTTGTTTCTTCCCATCAACCACTTAAATCTAACCTCTCTTTTACCAACCTACACCACTCCTTGTCCTAACGGCTCCTGGAGAGAAATGCGGCTAAGTACCACCGAGATTAGGACCTATAGTTCTCCTGTGGGCCCTTGTTTGGATCCTAACGACTTTACTATACACTGTAACTATGTATATAGCATCTCCGTCCTAATCGGTCAAAATAGGACTGATAATTCAAACCCTATCTTTGATACATAGGACGAGAACCTGACGTACTTAAACGACCACTCAATAGGTGTGAACTCAGGAACTACTTAGAAATGGTCGTTTACCCTCTTTGACGTGGGATGTACCATT-5'

Q2: mRNA sequence:

5'-ACCAAACAAAGUUGGGUAAGGAUAGAUCAAUCAAUGAUCAUAUUCUAGUACACUUAGGAUUCAAGAUCCUAUUAUCAGGGACAAGAGCAGGAUUAGGGAUAUCCGAGAUGGCCACACUUUUGAGGAGCUUAGCAUUGUUCAAAAGAAACAAGGACAAACCACCCAUUACAUCAGGAUCCGGUGGAGCCAUCAGAGGAAUCAAACACAUUAUUAUAGUACCAAUUCCUGGAGAUUCCUCAAUUACCACUCGAUCCAGACUACUGGACCGGUUGGUCAGGUUAAUUGGAAACCCGGAUGUGAGCGGGCCCAAACUAACAGGGGCACUAAUAGGUAUAUUAUCCUUAUUUGUGGAGUCUCCAGGUCAAUUGAUUCAGAGGAUCACCGAUGACCCUGACGUUAGCAUCAGGCUGUUAGAGGUUGUUCAGAGUGACCAGUCACAAUCUGGCCUUACCUUCGCAUCAAGAGGUACCAACAUGGAGGAUGAGGCGGACCAAUACUUUUCACAUGAUGAUCCAAGCAGUAGUGAUCAAUCCAGGUCCGGAUGGUUCGAGAACAAGGAAAUCUCAGAUAUUGAAGUGCAAGACCCUGAGGGAUUCAACAUGAUUCUGGGUACCAUUCUAGCCCAGAUCUGGGUCUUGCUCGCAAAGGCGGUUACGGCCCCAGACACGGCAGCUGAUUCGGAGCUAAGAAGGUGGAUAAAGUACACCCAACAAAGAAGGGUAGUUGGUGAAUUUAGAUUGGAGAGAAAAUGGUUGGAUGUGGUGAGGAACAGGAUUGCCGAGGACCUCUCUUUACGCCGAUUCAUGGUGGCUCUAAUCCUGGAUAUCAAGAGGACACCCGGGAACAAACCUAGGAUUGCUGAAAUGAUAUGUGACAUUGAUACAUAUAUCGUAGAGGCAGGAUUAGCCAGUUUUAUCCUGACUAUUAAGUUUGGGAUAGAAACUAUGUAUCCUGCUCUUGGACUGCAUGAAUUUGCUGGUGAGUUAUCCACACUUGAGUCCUUGAUGAAUCUUUACCAGCAAAUGGGAGAAACUGCACCCUACAUGGUAA-3'

Q3: Protein sequence:

MATLLRSLALFKRNKDKPPITSGSGGAIRGIKHIIIVPIPGDSSITTRSRLLDRLVRLIGNPDVSGPKLTGALIGILSLFVESPGQLIQRITDDPDVSIRLLEVVQSDQSQSGLTFASRGTNMEDEADQYFSHDDPSSSDQSRSGWFENKEISDIEVQDPEGFNMILGTILAQIWVLLAKAVTAPDTAADSELRRWIKYTQQRRVVGEFRLERKWLDVVRNRIAEDLSLRRFMVALILDIKRTPGNKPRIAEMICDIDTYIVEAGLASFILTIKFGIETMYPALGLHEFAGELSTLESLMNLYQQMGETAPYMV

Q4: Mutated mRNA:

5'-UGGUUUGUUUCAACCCUAUCCUUACUAGUUAGUUACUAGUUAAAGUACUAGUGAUACCUAAGUUCUAGGUAAUAAGUCCCUGUUCUCGUCCUAUACCCUUAAGGCUCUACCGGUGUGAAAACUCCUCGAUACGUAACAAGUUUUCUUUGUUCCUGUUUGGUGGGUAUAGUAGUCCUAGGCCACCUCGGUAGUCUCCUUAGUUUGUGUAUAAUAUACUAGGUUAAGGACCUCUAAGGAGUUAUAGGUGAGCUAGGUCUGUAGACCUGGCCAACCAGUCCAUAUAACCUUUGGGCCUACACUCGCCCGGGUUUGUAUGUCCCCGUGUAUUACCUAUAAUAAGGAUAAAACACCUCAGAGGUCCAGUUAACUAAGUCUCCUAGUGGCUACUGGGACUGCAUACGUAGUCCGACAUACUCCAACAAGUCUCACUGGUCAGUGUUAGACCGGAUAGGAAGCGUAGUUCUCCUAGGUUGUACCUCCUACUCCGCCUGGUUUAGAAAAGUGUACUACUAGGUUCGUCUACACUAGUUAGGUCCAGGCCUACCAAGCUCUUGUUCCUUUAGAGUCUUAAACUUCACGUUCUGGGACUCCCUAAGUUGUACUAAGACCCUAGGUAAGUACGGGUCUAGACCCAGAACGAGCGUUUCCGCCAUAGCCGGGGUCUGUGCCGUCGACUAAGCCUCGUAUCUUCCACCUUAUUCUAGUGGGUUGUUUCUUCCCUACAACCACUUAAUACUAACCUCUCUUUUACCAACCUACACCACUCCUUGUCCUAACGGCUCCUGGAGAGAAUAGCGGCUAAGUACCACCGAGUAUAGGACCUUAAGUUCUCCUGUGGGCCCUUGUUUGGUACCUAACGACUUUACUUAACACUGUAACUUAGUUAUAAGCUACUCCGUCCUAUACGGUCAAAUAAGGACUGUAAUAUCAAACCCUUACUUUGUAACUAAGGACGAGAACCUGACGUACUUAAACGACCACUCAUAAGGUGUGAACUCAGGAACUACUUAGAAUAGGUCGUUUACCCUCUUUGACGUGGGUAGUACCUAU-3'

Q5: Mutated protein sequence:

MSPCITYNKDKTPQRSS