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you briefly enter in an expert cave and shortly after emerging several hours later you begin to experience difficulty forcing your muscle to reduce the movements required for breathing and it’s hard to make your legs move so that you can walk what happened?


DNA polymerase I (Pol I) of E. coli consists of three functional parts (domains): an N-terminal domain with 5´ to 3´ exonuclease activities required for removal of the RNA primer, a central domain responsible for 3´ to 5´ exonuclease proofreading, and a C-terminal domain with polymerase activity. Pol I is thought to simultaneously remove RNA primers and fill in the gaps that result. A group of proteins known as RNaseH also have 5´ to 3´ exonuclease activity and can thus remove RNA primers. However, they lack the other two functions observed for Pol I. Predict the ability of the following mutants to replicate DNA:(1) a strain with a mutant gene encoding Pol I such that it no longer has polymerase activity (but retains both types of nuclease activities);   (2) a strain without RNaseH proteins; 


You can smell sulfur when boiling eggs. What amino acids do you expect in the egg?


DNA polymerase I (Pol I) of E. coli consists of three functional parts (domains): an N-terminal domain with 5´ to 3´ exonuclease activities required for removal of the RNA primer, a central domain responsible for 3´ to 5´ exonuclease proofreading, and a C-terminal domain with polymerase activity. Pol I is thought to simultaneously remove RNA primers and fill in the gaps that result. A group of proteins known as RNaseH also have 5´ to 3´ exonuclease activity and can thus remove RNA primers. However, they lack the other two functions observed for Pol I. Predict the ability of the following mutants to replicate DNA:

(1) a strain with a mutant gene encoding Pol I such that it no longer has polymerase activity (but retains both types of nuclease activities)


DNA polymerase I (Pol I) of E. coli consists of three functional parts (domains): an N-terminal domain with 5´ to 3´ exonuclease activities required for removal of the RNA primer, a central domain responsible for 3´ to 5´ exonuclease proofreading, and a C-terminal domain with polymerase activity. Pol I is thought to simultaneously remove RNA primers and fill in the gaps that result. A group of proteins known as RNaseH also have 5´ to 3´ exonuclease activity and can thus remove RNA primers. However, they lack the other two functions observed for Pol I. Predict the ability of the following mutants to replicate DNA:

(1) a strain with a mutant gene encoding Pol I such that it no longer has polymerase activity (but retains both types of nuclease activities);   

(2) a strain without RNaseH proteins;


DNA polymerase I (Pol I) of E. coli consists of three functional parts (domains): an N-terminal domain with 5 ´ to 3 ´ exonuclease activities required for removal of the RNA primer, a central domain responsible for 3 ´ to 5 ´ exonuclease proofreading, and a C-terminal domain with polymerase activity. Pol I is thought to simultaneously remove RNA primers and fill in the gaps that result. A group of proteins known as RNaseH also have 5 ´ to 3 ´ exonuclease activity and can thus remove RNA primers. However, they lack the other two functions observed for Pol I. Predict the ability of the following mutants to replicate DNA:
(3) a strain with a mutant gene encoding Pol I such that it no longer has 5 ´ to 3 ´ exonuclease activities (but retains 3 ´ to 5 ´ nuclease and polymerase activities);
(4) a strain with the mutant Pol I described in and a strain lacking all RNaseH proteins.
Explain your reasoning for each.
DNA polymerase I (Pol I) of E. coli consists of three functional parts (domains): an N-terminal domain with 5 ´ to 3 ´ exonuclease activities required for removal of the RNA primer, a central domain responsible for 3 ´ to 5 ´ exonuclease proofreading, and a C-terminal domain with polymerase activity. Pol I is thought to simultaneously remove RNA primers and fill in the gaps that result. A group of proteins known as RNaseH also have 5 ´ to 3 ´ exonuclease activity and can thus remove RNA primers. However, they lack the other two functions observed for Pol I. Predict the ability of the following mutants to replicate DNA:
(1) a strain with a mutant gene encoding Pol I such that it no longer has polymerase activity (but retains both types of nuclease activities); [3]
(2) a strain without RNaseH proteins;
  • If the number of chromosomes starts with 22,how many chromosomes and chromatids are there in the cell after telophase 2

Enzymes are protein catalysts; they influence the kinetics but not the thermodynamics of a reaction. Discuss.


Where are the receptors for sweet tasting molecules located on the tongue?


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