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You are interested to study the mutation in the regulatory gene (lach) of a lac operon which significantly alters the shape of repressor protein produced, causing loss of function. By using separate labelled diagrams, describe briefly the actions of the normal and mutated fac operon in absence of lactose.

As a laboratory researcher, you have obtained three (3) human gene sequences from the gene bank. Of which. they were found out to be template that encoded for peptide hormone A, hormone B and hormone C in human body. The production of these hormones can have pharmaceutical uses in the future, for example, hormone replacement therapy in treating disease. Expression of biological information is one of the important steps to produce exogenous protein in biotechnology field. To figure this out, you look into the process of protein synthesis that occurs naturally in a cell.




Gene sequence for hormone A:




3' ...TAC TGG ATG CGC CTC CTG CCC CTG GCG CCA ATT ... 5¹




Gene sequence for hormone B:




3'...TAC GTT ATG CCC ATA TAG TTT CCT GAG AAG ATC... 5'




Gene sequence for hormone C:




3' ...TAC TCC TTG CGG CTC GTA TCT CTG TCG CAA ACT... 5¹

Write on the following.


1. Extra terrestrial system.


2. Quorum sensing.

In a laboratory lesson in histology, a student studied a micropreparation at a low magnification of the microscope, and then wanted to examine the structures of interest at a high magnification, but, despite attempts to focus the image, he did not achieve clarity, and the glass of the specimen broke. What mistakes were made when studying the micropreparation?



3.If you are given to address one environmental problem, what would it be? Why?




i. What is the difference between Weak Sustainability and False Assumptions?




ii. There are some economic tools for sustainability that can be put into practice. Give at least four.

Why is Sustainability science regarded as Transdisciplinary?

Geographical distribution of organisms describes the way organisms are dispersed over the face of the earth. The biodiversity and distribution of organisms within an ecosystem is due to both abiotic and biotic factors. Elaborate on these two terms giving three examples of each.


What is population ecology and why is it easier to study plant population than animal

population? Explain why populations cannot continue to increase indefinitely?


In what order does the human body use it’s energy sources? Glycogen first, then fat and last muscle protein?


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