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Which of the following is the correct sequence of events in aerobic respiration?

b. Glycolysis, Citric Acid Cycle, Pyruvate Dehydrogenase Complex, Oxidative Phosphorylation

a. Citric Acid Cycle, Pyruvate Dehydrogenase Complex, Oxidative Phosphorylation, Glycolysis

d. Glycolysis, Pyruvate Dehydrogenase Complex, Citric Acid Cycle, Oxidative Phosphorylation

c. Glycolysis, Oxidative Phosphorylation, Citric Acid Cycle, Pyruvate Dehydrogenase Complex


The process by which carbon changes from carbon dioxide to glucose and back is called

a. Glycolysis

b. The Carbon Cycle

c. The Calvin Cycle

d. The light dependent reactions


ATP synthase is an example of an

c. Enzyme and Form of Energy

d. Enzyme only

b. Protein and Form of Energy

a. Enzyme and Protein


Most of the CO2 from aerobic respiration is released during? 

a. Glycolysis

d. Electron Transport Chain

b. Pyruvate Dehydrogenase Complex

c. Citric Acid Cycle



How many pyruvate molecules are generated by the glycolysis of 3 glucose molecules?

d. 12

a. 1

b. 3

c. 6


The immediate energy source that drives ATP synthesis during oxidative phosphorylation is

b. That attraction of electrons to Oxygen

a. The flow of electrons down the electron transport chain

c. The proton gradient created across the membrane

d. ATP from glycolysis


The stage of photosynthesis that uses the most ATP molecules is

c. Glycolysis

a. The Calvin Cycle

d. The electron transport chain

b. The light dependent reactions


A group of researchers at the U.S. Department of Agriculture isolate a new bacterium



from the lungs and lymph nodes of several young horses that became ill and died at a



local stable. Prior to death, their symptoms included disorientation and loss of motor



function, so the researchers suspected central nervous system involvement, which was



confirmed by the observation of brain lesions in the dead animals. Based on 16S rRNA



comparisons, the bacterium was distantly related to Neisseria meningitidis, and they



subsequently named it Neisseria equiniae. The researchers believe that N. equiniae may



be responsible for the brain lesions observed in a small percentage of older horses that die



of apparent dementia. What four criteria must be satisfied in order for the researchers to



prove that the brain lesions in these older horses are caused by N. equiniae? Provide at



least three modern molecular experiments that could be performed to satisfy these



criteria.


What mutation in the DNA sequence for a protein would make the protein more

likely to dissolve in aqueous solutions?


1.      70-75% ethanol is the most common disinfectant/sterilizing-sanitizing agent used in a microbiology laboratory. Explain why 70-75% ethanol is more effective than 95-99% ethanol. (5 marks).

2.      List TWO GLPs and discuss its importance. (5 marks).

3.      Why should Good Laboratory Practices be followed strictly? State TWO reasons. (5 marks).

4.What does ISO stand for? What is its significance? 


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