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A new disease associated with glycogen metabolism has been discovered. It involves a mutation in the glycogen phosphorylase and glycogen synthase enzymes where all of the serine residues in the active and regulatory sites are replaced with glycine residues. Assuming this has no effect on the actual catalytic mechanism of these enzymes describe all of the effects on regulation of glycogen metabolism that would occur.


Some dogs bark when trailing; others are silent. The barking trait is due to dominant alleles. Erect ears (tall ears) are dominant to drooping ears (small ears). What kind of pups would you expect from a heterozygous, tall ear barker mated with a dominant small ear silent trailer.



Can a cellular culture be sustained in an environment with constant neurotransmitters signals without neurodegeneration, if the cells were readily supplemented with a high contents medium?


What is the change in pH if 1.50mL of 1.00M HCl is added to the buffer solution? Is the buffer effective? Show your calculation.

They are some codes in retroviral RNA:

R, U5, gag, pol, env, PP, U3

What do they stand for?


Observe: Look at the SUMMARY tab. How many fish species are present now?


What is the definition of a tetrapodomorph fish?


A sample from the planet Mars has yielded a new eukaryotic organism, the MARS Amoeba (MARSA). Initial studies have determined it contains a nucleus with 3 distinct DNA based chromosomes. You have been assigned the task to study this new creature.

 

Using your knowledge, you are expected to: (25 points each)

 

1)   Generate both MARSA genomic and cDNA libraries while testing your libraries for completeness and titer by using many techniques discussed in our lectures such as Southern and Northern Blotting, mRNA to cDNA techniques, genomic DNA isolation via Principle of pulse-field gel electrophoresis (PFGE) followed by partial restriction digestions, and ligations into lambda phage vectors (expression-based Lambda gt11.). Include in your written journey all need steps and controls.


Mrs. CD is a 49 years female who has been admitted in hospital for her road-side accident. She has S. aureus infection for which she was administered with intravenous nafcillin at a dose of 2 grams every four hours. On discharge, she was handed over a prescription of orally oxacillin at a dose of 500 milligram four-times daily. After two weeks, she was again admitted in the ED because of her fever, body rashes, blood in urine, and reduced urine urgency. Her blood biochemistry revealed a blood urea nitrogen level of 34 mg/dL, serum creatinine level of 2 mg/dL, and elevated white blood cells count. Urinalysis showed the fractional excretion of sodium as 3% and a positive test for the presence of eosinophils, and red blood cells. What is the likely diagnosis and how she can be treated?

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