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  1. if you want to overexpress a protein in ecoli. The protein is from human ocular cells. What vector would you use to express the protein?. You know the sequence of the gene for the ocular protein, where would you get the DNA you need to clone into the vector? And what regulatory sequences need to be present in your vector for expression to occur?
  2. how will u make a conditional mutant in mice using cre/lox system?

thank you


Why is there uncertainty in breeding plants with a dominant allele?


Hi, in the olfactory system, does neurogenesis occur?


Explain how viscosity is related to the flow and attraction between atoms in a liquid.



Mammals, lungfish, ray-finned fish, avians, and squamates are each known as


Suppose you have an n×n additive matrix M with n ≥ 4, and you erase the entries

corresponding to two symmetric entries Mij and Mji in the matrix. Give an algorithm

to infer those two missing values (Mij and Mji) from the remaining data, and prove it

correct.


I want to know the general concentration of catalase in Brócoli, potatoes, onions, carrots and cucumbers


What would be the magnification of a specimen deal with a compound light microscope that has an objective power of 40 X and an ocular lens power of 10 X


1. Under aerobic catabolism of glucose, in which compartment of the eukaryotic cell does 

the following reactions occur?

a. Conversion of pyruvate to acetyl CoA.

b. Conversion of succinyl-CoA to succinate

c. Conversion of NADH to ATP

d. Conversion of phosphoglycerate to phosphoenolpyruvate


2. Describe various mechanisms for regulation of blood glucose.


3. Give an account of β – oxidation of saturated even carbon fatty acid (Palmitic acid)

along with the total ATPs obtained. 


Calculate the salt to acid ratio ([Aˉ]/[HA]) of a solution of an amino acid at pH 2 and 

pKa of 8.95


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