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John has met with an accident, where he is succumbed to a condition with multiple injuries like rib fracture, bone fracture leading to loss of leg
Based on the above scenario, the person ended up in breathing difficulty; suggest the role of respiratory system in this regard. (3 marks)
Because of breathing difficulty there is loss of oxygen levels in the body leading to unconsciousness. Suggest the role of different systems that are required to revive the patient’s conscious condition. (3 marks)
After discharge, the patient referred to rehabilitation center for bringing back to routine. What sort of engineering solutions would you adopt for his revival? (4 marks

Describe the His-Tag expression concept and Ni Affinity chromatography


1) If enzymes are converted into solid state, will they function...?


2) What will happen if electricity is pass through enzymes in vitro....?


1) Which enzyme is responsible for the absorption of CO2 from the air, in plants....?


2) What is the work of Rubisco enzyme...?



Does bacteriophage take back its DNA after bursting the bacteria?


Distinguish between the length of day and night during the following seasons:
a. Summer
b. Winter

How much quantity of Rubisco enzyme is required to absorb 2 tons of CO2 from the air in one day.....? (In grams)


bile pigments are waste products resulting from

a)destruction of old RBCs in the liver b)the action of hydrochloric acid c)the breakdown of fat d)activation of pepsinogen


describe activation of CD4 helper T cells by interaction of the TCR with peptide fragments presented by class II MHC molecules on APCs


Describe cross presentation of antigen in which dendritic cells are able to capture and ingest a virus infected or tumor cell and present the antigen to naive CD8 T cells via the class I MHC pathway


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