Biology Answers

1. The Michaelis-Menten equation is a mathematical model that is used to analyze simple kinetic data. The model has certain assumptions, and as long as these assumptions are correct, it will accurately model your experimental data. Using relevant stoichiometric equations, list and discuss all the assumptions from which the Michaelis-Menten equation is derived.

There are three broad ways in which enzyme activity can be regulated. Allosteric inhibition and covalent modification are two of the three ways.

Name one enzyme regulated each way. Give detail as appropriate for each means of

regulation. If external molecules or modifications are relevant, your answer must include

them and a description must include the effect the molecules or modifications have on

enzyme.

When looking at DNA replication what are the most important steps and enzymes used ?

What is the overview of reproduction in Eumycota?

what are the random and non-random events in sexual reproduction (post-meiosis) that ultimately determine the genetic makeup of the offspring?

Bryan is competing in a strongman competition tomorrow. He is heavily working out and needs to build and repair his muscles and also needs to load up on energy for tomorrow. What macromolecules should he be primarily consuming?

DNA polymerase I (Pol I) of E. coli consists of three functional parts (domains): an N-terminal domain with 5 ´ to 3 ´ exonuclease activities required for removal of the RNA primer, a central domain responsible for 3 ´ to 5 ´ exonuclease proofreading, and a C-terminal domain with polymerase activity. Pol I is thought to simultaneously remove RNA primers and fill in the gaps that result. A group of proteins known as RNaseH also have 5 ´ to 3 ´ exonuclease activity and can thus remove RNA primers. However, they lack the other two functions observed for Pol I. Predict the ability of the following mutants to replicate DNA:

) a strain with a mutant gene encoding Pol I such that it no longer has 5´ to 3´ exonuclease activities (but retains 3´ to 5´ nuclease and polymerase activities