Question

If absorbance is 0.486 and the extinction coefficient, molar absorbtivity is 36000 per molar per cm at 414 nm. And you take 100 microlitre of the given solution in a 1 ml cuvette with 900 microlitre buffer. What is the concentration of the given stock of ABTS?

Answer 1

Solution.

To find the concentration of the ABTS stock, it is necessary to write the Bouguer – Lambert – Beer Law and derive the concentration from this equation:

$$A = \epsilon \times l \times C$$

$$C = \frac{A}{\epsilon \times l}$$

$$C = \frac{0.486}{36000 \times 1}$$

$$C = 1.35*10^{-5} M$$

Answer:

$$C = 1.35*10^{-5} M$$