Answer to Question #91895 in General Chemistry for Dheepu Shree

Question

If absorbance is 0.486 and the extinction coefficient, molar absorbtivity is 36000 per molar per cm at 414 nm. And you take 100 microlitre of the given solution in a 1 ml cuvette with 900 microlitre buffer. What is the concentration of the given stock of ABTS?

Answer 1

Solution.

To find the concentration of the ABTS stock, it is necessary to write the Bouguer – Lambert – Beer Law and derive the concentration from this equation:

$$A = \epsilon \times l \times C$$

$$C = \frac{A}{\epsilon \times l}$$

$$C = \frac{0.486}{36000 \times 1}$$

$$C = 1.35*10^{-5} M$$

Answer:

$$C = 1.35*10^{-5} M$$