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Prokaryotic and eukaryotic are similar because the both have?


b)Show how the student would calculate the value of 1 ocular division in micrometres to two decimal places. (3 marks)













































d) Which part of the microscope associated with the mechanical stage is the specimen slide held? (1 mark)

Describe the His-Tag expression concept and Ni Affinity chromatography


Which kingdoms contain organisms with many cells


Avery and his colleagues purified various chemicals from pathogenic bacteria and showed that ________ was (were) the transforming agent.



Why IPTG is used for expression studies than lactose/ allolactose in E. coli?a. Concentration remains constant


The high copy number plasmid is isolated from 2 mL of E. coli cells containing 4 × 108 CFU/mL and it is quantified as 120 ng/µL using nanospectrophotometer. Size of the plasmid is 5400 kb. Convert the concentration of plasmid from ng to pM concentration.

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15. A researcher is working with haemoglobin that contains subunits of differing molecular weights. If the researcher performs SDS-PAGE, how many distinct bands should he see on the gel?


A researcher isolates DNA using TBE buffer and use it for cloning experiment. He fails to get the recombinant plasmid, why?


2.3) Which part of a ribosome accepts a tRNA molecule that is attached to a growing polypeptide chain?

  1. A site
  2. P site
  3. E site
  4. all because the growing polypeptide moves from one site to the other.
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