Question

In certain cats, tail can be long, manx (no tail), or in the case of the heterozygous condition, short. What is the probability that many offspring are produced if a short tailed cat is bred with a manx cat?v

Answer 1

Question #81879, Biology, Genetics

Question:

You order a PCR primer from Eurofins Genomics. They deliver a tube with 35 nmoles of DNA in it. How much TE buffer do you need to turn it into a 100 µM freezer stock? Which pipettor should you use? To what should it be set?

Solution:

Molar concentration can be calculated from the equation:

$$C = \frac{v}{V}$$

where C – molar concentration, v – number of moles, V – volume.

From the equation:

$$V = \frac{v}{C}$$

As the required molar concentration is C = 100 µM, v = 35 nmol, then:

$$V = \frac{v}{C} = \frac{35 \text{ nmol}}{100 \mu \text{M}} = \frac{35 \times 10^{-9} \text{ mol}}{100 \times 10^{-6} \text{M}} = 0.35 \times 10^{-3} \text{L} = 350 \mu \text{L}$$

Answer:

To make the required freezer stock, 350 µL should be added into a tube using a pipettor of the volume 1 mL set to 350 µL.