Biochemistry Answers

In a procedure for testing blood glucose, a drop of blood is placed on a paper strip impregnated with the enzyme glucose oxidase and all the reagents necessary for the reaction.

The H2O2 produced causes a color change on the paper that indicates how much glucose is present. Since glucose oxidase is specific for the beta anomer of glucose and doesn’t act on alpha anomer, in spite of this specificity, the reaction is used in clinical assay for measuring total blood glucose which consists of a mixture of beta D glucose and alpha D glucose. Explain how this assay

Why is the beta-D-glucopyranose form of glucose more abundant than alpha-D-glucopyranose in aqueous solution?

4. a. In a procedure for testing blood glucose, a drop of blood is placed on a paper strip impregnated with the enzyme glucose oxidase and all the reagents necessary for the reaction.

The H2O2 produced causes a color change on the paper that indicates how much glucose is present. Since glucose oxidase is specific for the beta anomer of glucose and doesn’t act on alpha anomer, in spite of this specificity, the reaction is used in clinical assay for measuring total blood glucose which consists of a mixture of beta D glucose and alpha D glucose. Explain how this assay is effective in measuring total blood glucose?

Polymerase chain reaction (PCR) is a

revolutionary method invented by Kary Mullis in

the 1980s which copies the target sequence

defined by a pair of primers exponentially. Can

exponential amplification of any random

sequence of DNA be achieved in PCR with only

single primer? Hint: Refer the schematic in the

diagram that represents randomly amplified

polymorphic DNA (RAPD) marker technology.

You can substantiate your answer with help of

this diagram, if required.

The BSA standard solution with the concentration of 0.5 mg/mL was used to produce standard curve for protein assay based on Bradford method. The test tubes contain BSA solutions was prepared: 0, 20, 40, 60, 80, 100 μL. The reading of the absorbance for each test tube after adding the Bradford reagent was 0, 0.05, 0.09, 0.14, 0.19 and 0.22. If 20 μL of protein solution with unknown concentration were taken and mixed with 80 μL water. Then, 10 μL of this mixture was reacted with Bradford reagent and the absorbance reading was 0.08. What is unknown protein solution concentration before dilution?

. A molecule of ATP is usually consumed within a minute after synthesis, and the average human adult requires about 65 kg of ATP per day. However, at any given instance, human body contains at max about 50 grams of ATP and ADP combined, how it is possible that so much ATP can be utilized? (4 marks)

(b) Does ATP have a role in energy storage? (3 marks)

A molecule of ATP is usually consumed within a minute after synthesis, and the average human adult requires about 65 kg of ATP per day. However, at any given instance, human body contains at max about 50 grams of ATP and ADP combined, how it is possible that so much ATP can be utilized?

at any given instance, human body contains at max about 50 grams of ATP and ADP combined, how it is possible that so much ATP can be utilized?

Draw a neat structure of Alanylglycylhistdyllysine

Two students performed the same experiment, testing how far iodine will diffuse through starchy tissue, such as potatoes. They cut 5 potato squares each, all of equal sizes, and placed them into small cups with iodine solution for 15 minutes. Then they measured how far from the edge of the potato the iodine diffused into the potatoes, in millimeters.





⎡⎣⎢⎢⎢⎢⎢Student 1Student 2Distance Diffused (mm)Trial 13.253.14Trial 23.173.43Trial 33.263.22Trial 43.643.37Trial 53.443.28⎤⎦⎥⎥⎥⎥⎥




What is the range of student 1’s results?


A. 3.29

B. 3.35

C. 0.47

D. 0.29