Biochemistry Answers

In aqueous solution, almost all D-glucose molecules are in the pyranose form. Other aldoses have a greater proportion of molecules in the open-chain form. D-Glucose may have evolved to be the predominant hexose because it is less likely than its isomers to react with and damage cellular proteins. Explain why D-glucose reacts less than other aldoses with the amino groups of proteins.

In a procedure for testing blood glucose, a drop of blood is placed on a paper strip impregnated with the enzyme glucose oxidase and all the reagents necessary for the reaction.

The H2O2 produced causes a color change on the paper that indicates how much glucose is present. Since glucose oxidase is specific for the beta anomer of glucose and doesn’t act on alpha anomer, in spite of this specificity, the reaction is used in clinical assay for measuring total blood glucose which consists of a mixture of beta D glucose and alpha D glucose. Explain how this assay

Why is the beta-D-glucopyranose form of glucose more abundant than alpha-D-glucopyranose in aqueous solution?

4. a. In a procedure for testing blood glucose, a drop of blood is placed on a paper strip impregnated with the enzyme glucose oxidase and all the reagents necessary for the reaction.

The H2O2 produced causes a color change on the paper that indicates how much glucose is present. Since glucose oxidase is specific for the beta anomer of glucose and doesn’t act on alpha anomer, in spite of this specificity, the reaction is used in clinical assay for measuring total blood glucose which consists of a mixture of beta D glucose and alpha D glucose. Explain how this assay is effective in measuring total blood glucose?

Polymerase chain reaction (PCR) is a

revolutionary method invented by Kary Mullis in

the 1980s which copies the target sequence

defined by a pair of primers exponentially. Can

exponential amplification of any random

sequence of DNA be achieved in PCR with only

single primer? Hint: Refer the schematic in the

diagram that represents randomly amplified

polymorphic DNA (RAPD) marker technology.

You can substantiate your answer with help of

this diagram, if required.

The BSA standard solution with the concentration of 0.5 mg/mL was used to produce standard curve for protein assay based on Bradford method. The test tubes contain BSA solutions was prepared: 0, 20, 40, 60, 80, 100 μL. The reading of the absorbance for each test tube after adding the Bradford reagent was 0, 0.05, 0.09, 0.14, 0.19 and 0.22. If 20 μL of protein solution with unknown concentration were taken and mixed with 80 μL water. Then, 10 μL of this mixture was reacted with Bradford reagent and the absorbance reading was 0.08. What is unknown protein solution concentration before dilution?

. A molecule of ATP is usually consumed within a minute after synthesis, and the average human adult requires about 65 kg of ATP per day. However, at any given instance, human body contains at max about 50 grams of ATP and ADP combined, how it is possible that so much ATP can be utilized? (4 marks)

(b) Does ATP have a role in energy storage? (3 marks)

A molecule of ATP is usually consumed within a minute after synthesis, and the average human adult requires about 65 kg of ATP per day. However, at any given instance, human body contains at max about 50 grams of ATP and ADP combined, how it is possible that so much ATP can be utilized?

at any given instance, human body contains at max about 50 grams of ATP and ADP combined, how it is possible that so much ATP can be utilized?

Draw a neat structure of Alanylglycylhistdyllysine