Biochemistry Answers

What is the significance of Iodine number?

State the importance of iodine in the diet of farm animals.

function of lysosomes

Function of Endoplasmic reticulum

How do you interpret the serum TSH measurement in the evaluation of a patient with suspected thyroid disease?

Outline the mechanisms involved in the regulation of calcium.

Lois, a 32-year old unmarried female business executive develops amenorrhea. She has not recently lost weight but states that her job is very stressful. Laboratory evaluation reveals the following results:



PARAMETER

VALUE

NORMAL RANGE

Serum estradiol

14pg/ml

23-145

LH

1.2mIU/ml

2-15

FSH

1.5mIU/ml

2-20

Prolactin

6.2ng/ml

2-25

TSH

1.2µU/ml

0.5-5.0


A serum pregnancy test is negative and an MRI scan of her pituitary gland is normal. What is the probable diagnosis? Explain.

1. Define pH and pKa. Use these concepts to predict the ionization state of any given amino acid or its side chain in a protein.

6) You create 40 mL of a 3.0 M buffer with a pH of 5. You add 7 mL of a 1.25M H2SO4 solution to the buffer you made, what is the new pH?


7) You create 50 mL of a 1.0M buffer with a pH of 2. You add 2 mL of a .45M HCl solution to the buffer you made, what is the new pH?


8) You create 30 mL of a 1.0M buffer with a pH of 7.5. You add 1.3 mL of a .66M HCl solution to the buffer you made, what is the new pH?


9) You create 100 mL of a 0.5M buffer with a pH of 6.8. You add 1.0mL of a 1.0M NaOH solution to the buffer you made, what is the new pH?


10) You create 100 mL of a 0.5M buffer with a pH of 11.75. You add 10.0 mL of a 1.0M HCl solution to the buffer you made, what is the new buffer [Base]?

1) You create 200 mL of a 0.35M buffer with a pH of 4.00. You add 8.0 mL of a 0.75M NaOH solution to the buffer you made, what is the new pH?


2) You create 200 mL of a 0.35M buffer with a pH of 4.00. You add 8.0 mL of a 0.75M HCl solution to the buffer you made, what is the new pH?


3) You create 50 mL of a 1.0M buffer with a pH of 4.7. You add 2 mL of a .45M NaOH solution to the buffer you made, what is the new buffer [Base]?

4) You create 150 mL of a 0.88M buffer with a pH of 3.3. You add 20.0 mL of a 0.35M HCl solution to the buffer you made, what is the new Buffer [Base]?


5) You create 50 mL of a 1.0M buffer with a pH of 4.7. You add 2 mL of a .45M NaOH solution to the buffer you made, what is the new buffer [Acid]?