Biochemistry Answers

Why does Reference strand-mediated conformation analysis (RSCA) needs a fluorophore on its probe? Is it because without the fluorophore probe, the signal (Etbr) would be too weak? Does more mismatches in the heteroduplex causes the ethylBr not bind well and give weak signal and may go undetected in the gel? And how do you order those heteroduplexes in the gel, more mismatches in the heterdoplux->less stable, so less migration and on top of the gel. Less mismatches, more stable heterduplex -> better migration on the bottom of the gel? http://imgur.com/0VP5bjq

Excess dietary fat can be converted into cholesterol in the liver. When Palmitate labeled with C14 at every even-numbered carbon is added to liver homogenate, where does the radiolabel appear in mevalonate?

name two inhibitors of glycolysis and explain their mechanism of action?

The pI value for amino acid cysteine is 5.1. Draw the structure of cysteine that predominates in a solution at each of the following pH values:
a. pH < 1
b. pH = 5.1
c. pH > 12

Use the three letter abbreviation to name the amino acid(s) having:
A. an alcohol functional group
B. an acidic side group
C. an a (alpha) amino group in a ring
D. a benzene ring

Which one of the following components of urine in a healthy human DOES NOT differ much in concentration from that of blood plasma? And why?
a) NH4+ b) K+ c) Na+ d) SO42-

If you had a solution of proteins from a crude extract in water, and added ammonium sulfate to 60% saturation, proteins with more hydrophobic surfaces exposed precipitate. If you then heated the solution to 60 degrees Celsius for 2 minutes, and then put the solution on ice, less-thermostable proteins will denature, and more more hydrophobic areas will be exposed on these proteins. Meanwhile, more-themostable proteins will not be denatured and remain in their native state. If you were designing a purification procedure to separate proteins on thermostability, after centrifuging, would you expect the less-theromostable proteins that are denatured to become less soluble and precipitate? Would this 60 degree Celsius heat treatment step cause more proteins to precipitate and become part of the pellet than before the step? How would you draw a flow chart for this step of a purification procedure?

Progestrone is pregnency mantaining hormone, then why it is used in contraceptive pills?

Does this statement true or not?(passive transport uses energy whereas active transport consumes energy in the form of ATP).

does this statement true or wrong (passive transport uses energy whereas active movement consumes energy in the form of ATP)and how can it be correct