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Separation of mixtures on a 3 metre column gave two peaks with retention times 14 and 17 minutes, base width of 1 minute for both peaks and dead volume of 10 minutes. Calculate column length to obtain a resolution of 1.5.

Hi,

I have been trying to solve one question in particular after our enzyme kinetics practical but I cannot seem to go any further. Would be able to help me out please. It’s been nearly 4 days now of me trying to solve it in different ways.

Q: Calculate the extinction coefficient (mM^-1.cm^-1) for the 0.5M ethanol (final concentration) sample that displays a maximum absorbance of 2.5. Assume full conversation of NAD+ (100microL in 2.4mL of 20MM solution)?

I appreciate that we have to use Beer-Lambert formula. Absorbance is 2.5, path length is 1.0. I cannot seem to find concentration of NADH using C1V1=C2V2, which will let me find extinction coefficient then.

Kind Regards

Draw the structure of the tripeptide, which is composed of Phe+Lys+Cys and answer additional questions .

1.1. specify the value of the isoelectric point

1.2 select the indicator and determine the pH of the aqueous solution by indicator's color 1.3 specify the hydrophilic-hydrophobic balance

1.4 draw the structures of the tripeptide that correspond to the pH values 0, pl, 7, 14. Specify the charge of the tripeptide in each pH

2. Construct the Fişcher's formula for the D-mannose [2(L),3(L),4(D),5(D),6- pentahydroxyhexan-1-al] and draw all the tautomers: oxo, and pyranoses, furanoses. 3.Give the formula of the nucleic base cytosine, nucleoside cytidine, nucleotide cytidine-3-phosphate

1. Draw the structure of the tripeptide, which is composed of Gly+Glu+Gln answer additional questions

.1.1 specify the value of the isoelectric point

1.2 select the indicator and determine the pH of the aqueous solution by indicator's color 1.3 specify the hydrophilic-hydrophobie balance draw the structures of the tripeptide that correspond to the pH values 0, pl, 7,

1.4. Specify the charge of the tripeptide in each pHAA

2. Construct the Fischer's formula for the D-idose (2(L),3(D),4(L),5(D),6- pentahydroxyhexan-1-al] and draw all the tautomers: oxo, and pyranoses,

3. Give the formula of the nucleic base cytosine, deoxynucleoside deoxycytidine, nucleotide deoxycytidine-5-phosphate furanoses.

Draw the structure of the tripeptide, which is composed of Ile+Lys+Asn and answer additional questions 1

. 1.1 specify the value of the isoelectric point

1.2 select the indicator and determine the pH of the aqueous solution by indicator's color 1.3 specify the hydrophilic-hydrophobic balance

1.4 draw the structures of the tripeptide that correspond to the pH values 0, pl, 7, 14. Specify the charge of the tripeptide in each pH

2 Construct the Fischer's formula for the D-galacose [2(D),3(L),4(L),5(D),6- pentahydroxyhexan-1-al] and draw all the tautomers: oxo, and pyranoses, furanoses.

3 Give the formula of the nucleic base thymine, deoxynucleoside deoxythymidine, nucleotide deoxythymidine-5-phosphate

Draw the structure of the tripeptide, which is composed of Ile+Asp+Met and answer additional questions

İ.1 specify the value of the isoelectric point

1.2 select the indicator and determine the pH of the aqueous solution by indicator's 1. color

1.3 specify the hydrophilic-hydrophobic balance

1.4. draw the structures of the tripeptide that correspond to the pH values 0, pl, 7, 14. Specify the charge of the tripeptide in each pH

2 Construct the Fischer's formula for the D- altrose [2(L),3(D),4(D),5(D),6- pentahydroxyhexan-1-al] and draw all the tautomers: oxo, and pyranoses, furanoses.

3 Give the formula of the nucleic base guanine, nucleoside guanosine, nucleotide guanosine -5-phosphate