Answer to Question #237765 in Biology for Agengo

Question

The composition of PBS is 0.137M Nacl, 0.012M KCL, pH 7.4. Below is the protocol to make 1litre of 10X concentration PBS. Combine the following

- 80g Nacl

- 2g Kcl

- 14.4g Na2HPO4( dibasic anhydrous)

- 2.4g KH2PO4(monobasic anhydrous)

-800ml distilled H2O

1.) Adjust pH to 7.4 with Hcl

2-) Add H2O to 1 litre

3.) Autoclave for 20 minutes on liquid cycle store at room temperature. Which ions are being produced by this process, assuming that each of the chemical compounds dissociate into their constituent parts once they are dissolved in water?