Molecular Biology Answers

Define the following:
Reading frame

Define the following:
Semiconservative replication

Discuss features of the eukaryotic cell cycle.

Explain the morphological changes in apoptosis.

Discuss control of transcription initiation by regulatory proteins.

3.1 Describe the process of cloning a DNA fragment into the PstI site of the vector pBR322.
How would you screen for clones that contain an insert?

Outline steps involved in DNA synthesis at the replication fork. How do DNA polymerase correct their mistakes?

1.3 Name and briefly discuss three important features of the genetic code

1.2 Draw the general structure of an amino acid and give the correct names for the four groups attached to the alpha-carbon of all amino acids

You are studying a protein that you suspect has DNA topoisomerase activity. Describe how you would test the protein for this activity in vitro.