Is there a methodology to deciding the correct/optimal substrate/medium in which a tissue culture should be grown. Or do prior tests need to be conducted to find the best medium type?
Gas levels in a cell culture can be measured using a gas analyser. How would you then control (increase/decrease) the amount of a gas in a cellular culture e.g. O2 or Co2?
I am working with an enzyme and need to inhibit the enzyme Acetylcholinesterase. Is there a methodology to take to select a given enzyme-inhibitor? Can you make any recommendations preferably the inhibitor should irreversible and non-competitive?
Post-synaptic transmission the enzyme Acetylcholinesterase (AChE) is produced to break-down acetylcholine. The enzyme AChE can be neutralised by the enzyme inhibitor AChEI, what is the concentration of AChEI in the synapse to neutralise all AChE enzyme? Does this differentiate between species?