NAD+ has negligible absorbable at 340nm. Using a name example, explain how you could use the change in absorbable properties between NADH (molar absorbable 6220M/cm) and NAD+ to measure the rate of a biochemical reaction.
Biological membranes are highly complex structures despite the mosaic of it's constituents. Explain how these chemically and physically diverse elements can form a coherent dynamic structure
180 µL of medium containing (200 µg/mL) Ampicillin were treated with 20 µL of ꞵ-lactamase enzyme to give finial concentrations ranging from 1:1 to 1:10 ratio of ampicillin to B-lactamase enzyme. The ꞵ-lactamase activity is 400 Unit/mg (Unit = 1 µmol / min hydrolysis of benzylpenicillin at pH 7 at 25C). The Ampicillin mass = 349.406 g/mol. Explain how to prepare the ꞵ-lactamase stock solution concentrations that can be used to give a final concentration ratio from 1:1 to 1:10 (ampicillin to enzyme) in the medium?
Which of the following buffers act on long term basis to regulate ph of blood: a) haemoglobin buffer b) bicarbonate buffer c)phosphate buffer d) ammonia buffer e) plasma proteins
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