Answer to Question #101045 in Biochemistry for Ryan Gamble

Question

NAD+ has negligible absorbable at 340nm. Using a name example, explain how you could use the change in absorbable properties between NADH (molar absorbable 6220M/cm) and NAD+ to measure the rate of a biochemical reaction.

Answer 1

In spectrophotometric methods, the progress of reaction is monitored by measuring the change in quantity of light the assay reaction absorbs. For example, in simple NAD/NADP .When lactate reacts with NAD+ in the presence of LDH the product is pyruvate and NADH and H+.As the amount of NADH reduces,UV absorbance will decrease