Answer to Question #101045 in Biochemistry for Ryan Gamble

Question #101045
NAD+ has negligible absorbable at 340nm. Using a name example, explain how you could use the change in absorbable properties between NADH (molar absorbable 6220M/cm) and NAD+ to measure the rate of a biochemical reaction.
1
Expert's answer
2020-01-07T05:11:25-0500

In spectrophotometric methods, the progress of reaction is monitored by measuring the change in quantity of light the assay reaction absorbs. For example, in simple NAD/NADP .When lactate reacts with NAD+ in the presence of LDH the product is pyruvate and NADH and H+.As the amount of NADH reduces,UV absorbance will decrease


Need a fast expert's response?

Submit order

and get a quick answer at the best price

for any assignment or question with DETAILED EXPLANATIONS!

Comments

No comments. Be the first!

Leave a comment

LATEST TUTORIALS
New on Blog
APPROVED BY CLIENTS