Biochemistry Answers

Why some scientists dehydrate enzymes before immobilization?

Treatment of a peptide with trypsin yields two fragments:

Leu-Ser-Tyr-Ala-Ile-Arg (LSYAIR) ; Asp-Gly-Met-Phe-Val-Lys (DGMFVK).

When the same peptide is treated with chymotrypsin, the fragments obtained are :

Val-Lys-Leu-Ser-Tyr ( VKLSY); Ala-Ile- Arg (AIR) ; Asp-Gly-Met-Phe (DGMF).

What is the sequence of this peptide ?

Isoelectric focusing is a technique for seperating amino acids.In a mixture of gly-gly-gly and gly-asp-glu, can gly-gly-gly be seperated from gly-asp-glu by isoelectric focusing ? Explain your answer.

1. Does the mitochondrial matrix ever run out of protons for pumping in oxidative phosphorylation?
2. Which of the following processes generates the most ATP? (Account for the no. of ATP)

a. ) Citric Acid Cycle

b.) Fatty Acid oxidation

c.) Glycogenolysis

d.) Glycolysis

e.) Pentose phosphate pathway

What is the name of the tiny balloon-like sacs?

1. Bronchi.

2. Alveoli.

3. Larynx.

4. Tracheas. 

Does the mitochondrial matrix ever run out of protons for pumping in oxidative phosphorylation?

Amanda is watching a fireworks display with her father. She loves watching the different colors of light as the fireworks explode in the air. Most things that give off light give off energy in what other form as well?

What is the overall chemical changes that occur during one complete turn of the Krebs cycle?

1. Which of the following processes generates the most ATP? (Account for the no. of ATP)

a.) Citric Acid Cycle

b.) Fatty Acid oxidation

c.) Glycogenolysis

d.) Glycolysis

e.) Pentose phosphate pathway

Destroying the Trojan horse. Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as β-lactamase), an enzyme present in some resistant bacteria. The mass of this enzyme in Staphylococcus aureus is 29.6 kd. The amount of penicillin hydrolyzed in 1 minute in a 10-ml solution containing 10^-9 g of purified penicillinase was measured as a function of the concentration of penicillin. Assume that the concentration of penicillin does not change appreciably during the assay.