Question

Treatment of a peptide with trypsin yields two fragments:

Leu-Ser-Tyr-Ala-Ile-Arg (LSYAIR) ; Asp-Gly-Met-Phe-Val-Lys (DGMFVK).

When the same peptide is treated with chymotrypsin, the fragments obtained are :

Val-Lys-Leu-Ser-Tyr ( VKLSY); Ala-Ile- Arg (AIR) ; Asp-Gly-Met-Phe (DGMF).

What is the sequence of this peptide ?

Answer 1

His-Ser-Asp- Ala-Ile-Phe-Thr-Gln-Gln-