Biochemistry Answers

Draw the structure of the tripeptide Ser-His-Asn in the ionic form that predominates at

pH 7. Show all atoms (including hydrogens) as well as any charges as necessary.

Explain the mode of action of fluoride and bisulphate on the fermentation pathway. Predict their likely effect if the yeast treated with these agents had been respiring.

make a detailed schematic diagram of the classification of lipids classification by function classification by structure

make a detailed schematic diagram of the classification of lipids classification by function classification by structure

Adenosine triphosphate, a peptide (1 kDa) and Green Fluorescent Protein (27 kDa) were mixed in solution and analysed simultaneously using native mass spectrometry. Assume that the three components are all ionised equally and transmit equally into the mass spectrometer. Sketch what the mass spectrum of this mixture would look like, providing an explanation for your answer.

How deficiency of riboflavin affects other vitamins especially vitamin B

• 2) Why are the ratios of OD260/OD280 and OD260/OD280 for clean nucleic acids about 2.0? Show your answer by drawing and explaining a DNA absorbance spectrum from 200 to 300 nm. Which type of contaminations can you detect with these measurements? 

In bacterial cell the DNA is the ?

Show your complete solution to earn points. Glucose, a major energy-yielding nutri￾ent, is present in bacterial cells at a concentration of about 1.6mM (mM=millimolar). 

What is the concentration of glucose, expressed as mg/mL? How many glucose mol￾ecules contained in a typical E. coli cell? (Recall Avogadro’s number= 6.023x1023)

Given: Glucose concentration= 1mM= 1x10-3 mol/L, Molecular weight=180g/mol, Vol￾ume= 1x10-15 L

yeast cells can catabolise glucose under both aerobic conditions (respiration) and anaerobic conditions (fermentation/glycolysis). In this experiment, we will study both processes using a culture of Saccharomyces cerevisiae which has been aerobically adapted. By relating the amount of ethanol produced to the amount of glucose consumed over the same period of time, we will attempt to determine which process is the most efficient provider of energy to the yeast cells. This assumes of course that the energy needs of the cells are similar under the two sets of conditions. 

how can we determine which process is more efficient by calculating Moles ethanol produced per mole glucose catabolized ??