Question

Adenosine triphosphate, a peptide (1 kDa) and Green Fluorescent Protein (27 kDa) were mixed in solution and analysed simultaneously using native mass spectrometry. Assume that the three components are all ionised equally and transmit equally into the mass spectrometer. Sketch what the mass spectrum of this mixture would look like, providing an explanation for your answer.

Answer 1

When the mixture Is analyzed using mass spectrometer a number of prominent peptides will be identified.