Answer to Question #110515 in Chemistry for Kesha Schaefer

Question

I cannot figure out how to upload the excel file.

a. This spreadsheet contains the digital data of six HPLC chromatographic injections (at different flow rates) of the same, two-component mixture through a 15.0 cm, reverse-phase column. For each chromatogram, determine the following;

Peak width at baseline for each peak, w.
Retention time for both peaks, tR.
Adjusted retention time for each peak, tr’.
Capacity factor for both peaks, k’.
Unadjusted relative capacity factor, γ.
Resolution between the peaks, R.


Summarize your results in the table below but you must also submit your work to get full marks.

Flow rate mL/ min 0.05 0.15 0.25 0.50 0.75 1.00
tm / secs. 1008 336 202 101 67 51
w / min
(1st peak)
w / min
(2nd peak)
tR / min
(1st peak)
tR / min
(2nd peak)
tR’ / min
(1st peak)
tR’ / min
(2nd peak)
k’ (1st peak)

k’ (2nd peak)

γ

R

Answer 1

Dear Kesha Schaefer, your question requires a lot of work, which neither of our experts is ready to perform for free. We advise you to convert it to a fully qualified order and we will try to help you. Please click the link below to proceed: Submit order