Organic Chemistry Answers

. A solution containing egg albumin (pI=4.6), β-lactoglobulin (pI=5.2), and chymotrypsinogen (pI=9.5) was loaded onto a column of diethylaminoethyl cellulose (DEAE-cellulose) at pH 5.4.The column was then eluted with a pH 5.4 buffer, with an increasing salt concentration. Predict the elution pattern. 

Write the Lewis and Kekule structures of the following (show your solutions following the steps):

i. NH₂OH

ii. CH₃CH₃

iii. CH₃OH

iv. CH₃NH₂

v. CH₃Cl

vi. CH₃SH

vii. H₂O

viii. CO₃^(-)

ix. H₃O ⁽⁺⁾

x. NO₃^(-)

a.) Complete the following chemical formula based on the valence electrons and electronic configuration. Make sure to follow the octet rule. Answer with solution please. Thank you :)

i. CCl_?

ii. CH_?Cl₂

iii. SiF_?

iv. CH_?O

v. CH₃CH_?

vi. CH_?NOH_?

vii. CH_?NNH_?

viii. CH_?NO

ix. CH_?NH

x. HNNH_?

a.) Complete the following chemical formula based on the valence electrons and electronic configuration. Make sure to follow the octet rule. Answer with solution please. Thank you :)

i.      CCl_?

ii. CH_?Cl₂

iii. SiF_?

iv. CH_?O

v. CH₃CH_?

vi. CH_?NOH_?

vii. CH_?NNH_?

viii. CH_?NO

ix. CH_?NH

x.     HNNH_?

b.) Write the Lewis and Kekule structures of the following (show your solutions following the steps):

i. NH₂OH

ii. CH₃CH₃

iii.    CH₃OH

iv. CH₃NH₂

v. CH₃Cl

vi. CH₃SH

vii. H₂O

viii. CO₃^(-)

ix.    H₃O ⁽⁺⁾

x.     NO₃^(-)      

In an enzyme reaction experiment, the concentration of the substrate S

S is varied and the velocity measured for each concentration, giving the following data.

[S](mM)= 0.2, 0.4, 0.6, 0.8, 1.0

V(μM/min)= 0.38, 0.62, 0.79, 0.91, 1

By sketching the Lineweaver-Burk plot of 1/V against 1/[S] determine an approximation of

Km and Vmax. No need to sketch.

Which of the following is the best approximation of Km and the maximum velocity Vmax?

How will you prepare 100 ml of 0.20M sodium chloride solution?

In what direction (toward the anode, toward the cathode, or toward neither) will the following 

proteins move in an electric field?

a. Serum albumin (pI=4.9) at pH 8.0

b. Urease (pI=5.0) at pH3.0 and pH 9.0

c. Ribonuclease (pI=9.5) at pH 4.5, 9.5 and 11.0

Calculate the solubility of NiCO3 in moles per liter and grams per liter (Ksp = 1.4 x 10-7)

A reversible chemical reaction combines A and B to produce C and D. At equilibrium, A, B and C are present in concentrations 1 M, 0.05 M, 500 mM respectively.

If the rate constants are k1=2M^{-2}s^{-1} and k2 = 1 M^{-1} s^{-1}, what is the concentration of D in M at equilibrium? Give the result to three significant figures.