Question

a chromatography experiment was done by injecting a mixture of two components, X and Y onto a chromatographic column with 2.00 X 10^-3 theoretical plate (N). the following results were obtained.

parameter dead time Component X Component Y

capacity factor - 3.50 5.45

retention time 3.33 min 15.00min 21.5 min

calculate

1. width at the base, for each peak
2. the resolution between component X and Y

Answer 1

1. 1.7 x 3.5 x1/2 = 2,975.

1.7 x 5.45 x1/2 = 4,6325.

2. 2.975 x 4.6325 = 13,7816875.