Answer to Question #89551 in Biology for Rachel

Question

An experiment is investigating the effect of an enzyme on a substrate in a buffered solution. Which of the following is an example of an appropriate negative control condition?
A) Substrate and buffer
B) Substrate, buffer and a different enzyme

Answer 1

A) Substrate and buffer.

Negative control represents a reaction where the enzyme is absent, or it is not working. The reaction should be characterized by the absence of any changes in the substrate concentration. As a result, the substrate in the buffer solution without any enzyme must be used as a negative control.