Question

One of the following is not a procedure to stain bacteria for examination with light microscope

Answer 1

Classic Gram staining techniques involves following steps:
1. Fixation of clinical materials.
2. Application of the primary stain (crystal violet).
3. Application of Mordant.
4. Decolorization Step.
5. Application of counter stain (safranin).
All other possible procedures are not relevant to staining bacteria for examination with light microscope.