When an egg comes into contact with the single sperm cells, the concentration of calcium in the cytoplasm increases. Calcium induces the release of cortical granules and blocks polyspermy, reactivates cell cycle, and stimulates the pronucleus formation. As a result, the absence of calcium will lead to polyspermy (the fertilized egg will contain a higher number of chromosomes). On the other hand, the lack of calcium will block the progression of the cell cycle and egg cells will not proliferate while the embryo will not be formed.
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