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1. Where do biotechnologist get a gene of interest?

2. Give one gene of interest and cute it's source.

3. How do the sticky ends of the bacterial plasmid and the target gene attract each other to form hydrogen bonds?

4. What is the difference between electroporation and particle gun method in relation to introducing the target gene into a host cell?

5. How do biotechnologist select and screen the bacterial cells for the presence of the target gene?
1. What are the two types of restriction enzymes?

2. Why is Taq polymerase used in PCR in replacement to DNA polymerase?

3. What role does reverse transcription play in testing samples from patients for COVID-19?
1. How do bacteria protect their own DNA from their restriction enzymes?

2. In case of amount of DNA fragments when are the following DNA cloning vectors are used: a. Bacterial plasmids , b. Bacteriophages,cosmids, and yeast artificial chromosomes (YAC)

3. Restriction enzymes cut DNA at specific sequences known as _________.
1. Cite three major differences RDT and classical breeding

2. How is RDT changing the world?
How did the lack of sea urchins change the effect of lionfish on the reef?
Critically describe the relationship between cells, tissues, organs and organ system
When approaching denatures the hydrogen bonds are broken. What levels of protein structure would this affect choose all that apply.
A. 1°
B. 2°
C. 3°
D. 4°

Describe the actions of our immune response. what is an antigen? what does it do?


If you were given a wilted, potted plant, how would you determine whether it was in a state of incipient wilting?

What is the molarity of a 250.0 mL aqueous solution that contains 18.7 g of AgNO3

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