Molecular Biology Answers

How to calculate the molecular mass and length of a segment of B-DNA specifying a 40-kD protein?

Six fragments are produced when DNA from the bacterial virus,  is cut with Hind III. If you were to cut human DNA with Hind III, would you expect to get more than 6 fragments or fewer than 6 fragments? Explain your answer.

rna _______ is an enzyme involed in transcription

I have some laboratory questions, I need to attach the laboratory description document.
Discussion
o What can you deduce from your experiment?
o What does your experiment tell you about your GOI?
o Is there anything that you could change to make a better experiment?
• For this, you can ALWAYS make a better experiment!
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o How could you extend this experiment to test out other aspects of the signaling module your GOI is either a part of or somehow interacts with?
Conclusions
o Provide a concluding, summary slide that depicts what you believe the “take home message” of your presentation is

Explain how cross-contamination can occur in laboratories and how we might minimise this.

You generate a mutant form of Ran that can not bind NTF2, but is capable of performing all other Ran related activities . if you express a GFP - tagged version of this protein in cells, predict where the protein will accumulate over time and indicate what Nucleotide- bound state it will be in .

Why do we need to isolate individual cells and grow them in culture?

What environmental factors do we need to control to maintain cell viability?

Design a set of Prime that can be used to PCR amplify the target DNA sequence indicated by the dots?

5’ GGCTAAGATCTGAATTTTCCGAG......TTGGGCAATAATGTAGCGCCTT 3’
3’ CCGATTCTAGACTTAAAAGGCTC.......AACCCGTTATTACATCGCGGAA 5’

If a single DNA molecule is amplified by PCR, how many DNA molecules will there be after 6 cycles?