Answer to Question #1650 in Molecular Biology for Emily

Question #1650
Design a set of Prime that can be used to PCR amplify the target DNA sequence indicated by the dots?

5’ GGCTAAGATCTGAATTTTCCGAG......TTGGGCAATAATGTAGCGCCTT 3’
3’ CCGATTCTAGACTTAAAAGGCTC.......AACCCGTTATTACATCGCGGAA 5’

If a single DNA molecule is amplified by PCR, how many DNA molecules will there be after 6 cycles?
1
Expert's answer
2011-05-18T04:56:30-0400
The forward and reverse primers would be:
5’…GGCTAAGATCTGAATTTTCCGAG......TTGGGCAATAATGTAGCGCCTT …3’
CCGATTCTAGACTTAAAA
---------------------------------------------------------------------
GCAATAATGTAGCGCCTT
3’…CCGATTCTAGACTTAAAAGGCTC.......AACCCGTTATTACATCGCGGAA… 5’
---------------------------------------------------------------------
After one cycle of PCR we get 2 molecules of DNA. So after 6 cycles we get 26 = 64 DNA molecules.

Need a fast expert's response?

Submit order

and get a quick answer at the best price

for any assignment or question with DETAILED EXPLANATIONS!

Comments

No comments. Be the first!

Leave a comment

LATEST TUTORIALS
New on Blog
APPROVED BY CLIENTS