Molecular Biology Answers

6) The biosynthesis of the polypeptide involved t-RNA with anticodons UUA, GGC, CGC, AAG, CGU. Determine the nucleotide sequence of the region of each strand of the DNA molecule that carries information about the synthesized polypeptide, and the number of nucleotides containing A, G, T, C in a double-stranded DNA molecule.

5) The translation process involved 30 t-RNA molecules. Determine the number of amino acids that make up the synthesized protein, as well as the number of triplets and nucleotides in the gene that encodes this protein.

4) Insulin protein - 51 amino acids. Find the number of nucleotides contained in the gene in which the insulin protein is programmed?

3) It is known that a certain gene of a eukaryotic cell contains 4 introns (two of 24 nucleotides and two of 36 nucleotides) and 3 exons (two of 120 nucleotides and one of 96 nucleotides). Determine: the number of nucleotides in the mRNA; number of codons in mRNA

2) The gene consists of 3 identical exons and 4 identical introns of the regions, and the introns consist of 120 nucleotides each, and the entire gene has 1470 nucleotides. How many codons will the pre-mRNA, each exon, mRNA have?

1) Given the sequence of RNA nucleotides. Determine the coding and template chains in the DNA molecule. Indicate the 5'-3 'ends of the RNA and DNA molecule. m-RNA fmet-G-AUGUUUCUACACACGGUAGACGCCGGCUGGCGAUAG - (A)

The solution of situational problems.

RNA polymerase recognition site -5’ TATATT 3’, -35 nucleotide sequence – 5’ TTGACA 3’

The site of recognition of the repressor protein in the operon (operator) is ATTTTAT, structural genes begin with TAC codons and end with ATC codons, TTTT spacer sites are located between the genes.

a) Using this data, construct a Lac-operon model consisting of three structural genes: the Z- structural gene consists of 5 codons, the Y and A structural genes are 4 codons.

b) Perform a transcription. How much RNA is formed from this site?

c) How many proteins are encoded in this DNA region? Define their structure.

4. A woman who has had rubella (a viral infectious disease) has a deaf child. What type of mutation are we talking about? Explain.

3. A 12-year-old boy has been diagnosed with a sarcoma of the lower jaw. The boy's parents are healthy. What mutation underlies this mutation?

3. A 12-year-old boy has been diagnosed with a sarcoma of the lower jaw. The boy's parents are healthy. What mutation underlies this mutation?