Answer to Question #105640 in Molecular Biology for Kelly

Question

ATGGGCGTTACAGGAATATTGCAGTTACCTCGTGATCGATTCAAGAGGACATCATTCTTT
CTTTGGGTAATTATCCTTTTCCAAAGAACATTTTCCATCCCACTTGGAGTCATCCACAAT
AGCACATTACAGGTTAGTGCAATTGGTGGACAGGATGGAGACAATGGATACCGGCAGGTATTGGAGTTACAGGCGTTATAATTGCAGTTATCGCTTTATTCTGTATATGCAAATTTGTCTTT(TAG)

• Restriction endonuclease sites:
Nde I - 5’-CATATG-3’
Xho I - 5’-CTCGAG-3’


Q1.) According to the primer design rules, what is the sequence of the shortest forward primer that could be used to amplify the glycoprotein-encoding gene for cloning into a pET-vector for expression of a C-terminally hexahistidine-tagged recombinant glycoprotein protein in Escherichia coli?

Please explain it step by step so I can understand better thank you.

Answer 1

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