Answer to Question #105639 in Molecular Biology for Kelly

Question #105639
What Tm value (to 1 decimal place) would you use for the forward primer when calculating the annealing temperature for the last 25 cycles of PCR? Assume the forward primer sequence is 5’ CAT ATG GGC GTT ACA GGA ATA TTG 3’.

Do show your calculations, provide the answer with units if possible.
1
Expert's answer
2020-03-18T09:16:00-0400
Dear Kelly, your question requires a lot of work, which neither of our experts is ready to perform for free. We advise you to convert it to a fully qualified order and we will try to help you. Please click the link below to proceed: Submit order

Need a fast expert's response?

Submit order

and get a quick answer at the best price

for any assignment or question with DETAILED EXPLANATIONS!

Comments

No comments. Be the first!

Leave a comment

LATEST TUTORIALS
New on Blog
APPROVED BY CLIENTS