Microbiology Answers

List any advantages of a negative stain over a simple stain.

What is 'cell'?

Why gargles should not be swallowed but held in the throat?

You are provided with agar held at 45 degrees celsius. Why is this temperature critical to the success of the experiment? (hint: 40 degrees celcius would be too low and 50 degrees celsius would be too high a temperature

The organelles called ________ are also known as the powerhouse of the cell.

Sugar is one of the main sources of ________ for cells.

Why are the abiotic and biotic factors different at high tide and low tide in a mangrove ecosystem?

Would increasing RepA production increase copy number? (2 marks)
1. Yes, because RepA in this system is needed to increase initiation at the ori.
2. No, because RepA in this system is involved in “handcuffing”.

Create an E. coli cell that does not allow for replication of the incA-containing P1 plasmid,
but still replicates its own DNA normally. (5 marks)
1. Add a gene to the chromosome that expresses an iteron-binding protein at high levels.
2. Add a gene to the chromosome that expresses a RepA binding protein that prevents RepA from
binding to the inc sites.
3. Add a gene that encodes a countertranscript to repA mRNA.
4. Add a gene that binds the host DnaG primase, inactivating it