Question

Create an E. coli cell that does not allow for replication of the incA-containing P1 plasmid,
but still replicates its own DNA normally. (5 marks)
1. Add a gene to the chromosome that expresses an iteron-binding protein at high levels.
2. Add a gene to the chromosome that expresses a RepA binding protein that prevents RepA from
binding to the inc sites.
3. Add a gene that encodes a countertranscript to repA mRNA.
4. Add a gene that binds the host DnaG primase, inactivating it

Answer 1

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