Question

What roles do the following play in cloning a DNA fragment?
(a) Restriction enzymes.
(b) Vectors.
(c) DNA ligase.
(d) Electroporation.

Answer 1

a. Restriction enzymes are used for isolation of specific gene, which will be cloned. They are also used for the opening of vector, within which the target gene will be inserted.
b. Vectors are used for delivery of the target gene into the cell, where cloning will be performed.
c. DNA ligase is used for the formation of bond between target gene and vector.
d. Electroporation is the method, by means of which entrance of DNA into the target cells is provided.