Answer to Question #103749 in Genetics for josh Dowling

Question #103749
I have a strain of MG1655 E.coli. It has a Cm cassette inserted into my locus of interest via lambda red approach. How can this strain be used to generate a PCR fragment with a high chance of recombination with a CFT073 strain genome?
1
Expert's answer
2020-03-02T06:17:40-0500
Dear josh Dowling, your question requires a lot of work, which neither of our experts is ready to perform for free. We advise you to convert it to a fully qualified order and we will try to help you. Please click the link below to proceed: Submit order

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