1) Acetyltransferase enzymes modify a lysine side chain with an acetyl group. Titration of the pKa of the lysine substrate reveals the pKa value to be 8.1. What is the likely environment surrounding the lysine sidechain before it is acetylated? (Choose all that apply)
Cannot hypothesize based on the given information and prior knowledge
Water accessible active site in the enzyme
Water inaccessible highly polar active site
Largely non-polar and water inaccessible active site
Solvent exposed and near the enzyme surface