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Answer to Question #57207 in Biochemistry for Sanjukta Ghosh
Question #57207
During Beta-oxidation in the first step R-CH2-CH2-CO-SCoA (Acyl CoA) is converted into R-CH2-CH=CH-CO-SCoA(Trans-delta2-enoyl CoA) with the conversion of FAD intoFADH2.
My question is are the two electrons and protons needed for reduction of FAD provided by Acyl CoA for Acyl CoA can only loose a H- and a H+ to become unsaturated, Trans-delta2-enoyl CoA,?
My question is are the two electrons and protons needed for reduction of FAD provided by Acyl CoA for Acyl CoA can only loose a H- and a H+ to become unsaturated, Trans-delta2-enoyl CoA,?
Expert's answer
Yes. In the first stage of Beta-oxidation two electrons and protons are needed for reduction of FAD.
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I got it Thank you.
Though FAD reduction requires two electrons and protons, only one electron and one proton are accepted by FAD. Acyl-CoA loses two protons and electrons. This is a two step reaction. The first hydrogen being removed by the active center base Glu376-COO- (Acyl-CoA dehydrogenase) as a H+. The second proton is then transferred as a hydride (H-) to the flavin N(5) position. Now, Acyl-Coa becomes unsaturated. FAD gets its seconf proton from the active center of Acyl-CoA dehydrogenase and becomes reduced to FADH2.
Thank you. But are the two electrons and protons needed for reduction of FAD provided by Acyl CoA ? Where does the 2nd electron come from as Acyl CoA can only looses a H- and a H+ to become unsaturated, Trans-delta2-enoyl CoA.
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